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Artificial Insemination in an Appaloosa Breeding Program
By Stephanie Donaldson

Originally Published in the Spring 1999 ICAA REPORT
 

A person with one or two mares can make a contribution to the recovery of our beloved Appaloosa breed by carefully selecting a stud, or studs, which have the best pedigree to cross with her mare. I, for example, want to do what I can for the Appaloosa. However, having only one mare and limited resources, I do not want to ship my mare long distances and to have her away from home for the long periods of time necessary to breed, ultrasound, and possibly re-breed her. If a person has many mares, shipping them may be out of the question due to cost and difficulty. It may not be possible to keep several stallions on the premises and there may not be many nearby.

The Advantages
In any case, using shipped semen is a very attractive approach. First, the mare stays at home or only goes as far as the nearest veterinary hospital or breeding facility. Second, the choice of stallions is almost unlimited, provided the stallion owners have this service available. Third, there is less chance of sexually transmitted diseases, or injury to mare or stallion by a kick. Finally, if the stallion is always handled by the same veterinarian or technician for semen collection, he can learn that stud-like behavior is allowed only under certain circumstances (special halter, handler, etc.). He can be taught that this behavior is not allowed with his usual handler and tack.

Advantages to having the mare stay at home are many. The inconvenience of shipping the mare is avoided. She is less stressed, and more likely to conceive and to carry her foal to term. She can continue to be ridden and kept physically fit, which also contributes to fertility. If she has a foal at her side at the time of breeding, the foal is not stressed by shipping to a strange environment, and its training can continue uninterrupted.

Using AI widens one's choice of stallions and therefore reduces the likelihood that significant inbreeding will occur. With too small a selection of stallions, gradually all one's mares become closely related to the studs usually used. By using shipped semen one can be more particular about stud choice, carefully studying pedigrees and photographs or videos, talking to breeders, and making the best selection possible, without being geographically limited. On can breed to that gorgeous stud clear across the continent, as seen advertised in the ICAA Report, or on our web site. Hopefully, one can optimize the chance of getting that perfect foal with that great pedigree, and just the color and pattern one has been wishing for.

The Disadvantages
There are disadvantages to AI as well. These include cost, timing, registry regulations, and, in some cases, reduced fertility of the stallion. Also, many stallion owners do not offer the option of shipped semen.

There is often an additional fee for semen collection and the shipping container, on top of the stud fee. Usually, most of this additional fee is refundable upon timely return of the reusable container. (If it is not returned in time, somebody else may not get their shipment in time.) Veterinary fees are considerably higher for breeders choosing AI, as it requires multiple barn calls for palpation, ultrasound, sedation, insemination, etc. Other costs include permits from registries and blood tests and/or DNA tests.

Scheduling AI can be tricky. If the mare does not have obvious and regular heats, she may be prepared by using hormones, administered orally and by injection, it induce ovulation. The veterinarian will determine the hormone schedule and check the mare's response. He or she will judge the stage of her ovulation cycle by ultrasound or by rectal palpation of her ovaries. A skilled veterinarian can feel a mare's ovaries and predict time of ovulation to within a day or less. As soon as one knows when the mare is due to ovulate, it is time to telephone the stallion owner with the exact date the semen will be needed. The timing of the semen shipment must coincide with the mare's ovulation, as the semen is only viable for one to three days. (If ovulation is to be induced, semen shipment is best planned for a weekday, as many shippers don't deliver on a weekend. Some shippers will deliver to the nearest airport on weekends, but it is necessary to go and pick it up.) On the day of insemination, there is another veterinarian visit for sedation, insemination and another hormone injection.

Some stallions may be less fertile using shipped semen than natural coverage. A mare owner has to make sure the stallion has high sperm motility and a good record of settling mares via shipped semen. The stallion should have been tested by a veterinarian for sperm concentration, motility and general appearance after a sample has been collected, extended, cooled, packaged and stored for 24, 48, and 72 hours. This should be checked at the time of each collection. It is a good idea for the mare owner to request a written report of this test.
Sperm motility always decreases with the passage of time during shipping, but this is more of a problem with some stallions that with others. The mare owner's veterinarian should evaluate the viability of the sample at the time of insemination. Again, this should not be a problem if both veterinarians check the semen, and if the stallion has been properly tested.

Registry Requirements
The requirements of the ICAA for breeding via shipped semen are as follows: The mare owner must apply for ICAA approval prior to insemination. The ICAA reserves the right to refuse approval if a suitable stallion stands within a reasonable distance. The stallion must be licensed with the ICAA. Both the stallion and the mare must be blood typed prior to the procedure. The resulting foal must also be blood typed. The mare must be listed as artificially inseminated on both the breeder's certificate and the stallion report. The stallion's license number must appear on the stallion report, along with the mare's name and registration number. Licensed veterinarians must collect, prepare, and ship the semen, as well as receive it and inseminate the mare. Both veterinarians must file a notarized statement with the ICAA, testifying to the procedure. (These rules are undergoing revision to streamline the process.)

In the case of the Appaloosa Horse Club, a foal resulting from artificial insemination using transported semen may be registered, but only if the stallion is ApHC registered. Also, the stallion owner must be issued an annual Transported Semen Permit. The stallion, the mare and the resulting foal must all be DNA typed to verify parentage. (DNA testing is simpler, less expensive, and more accurate than blood typing. It involves pulling a few man hairs out by the roots, or doing a nasal swab, and sending it to a lab for analysis. Both DNA typing and blood typing only need to be done once for each horse; the results are then on file for life. Kits for both methods of typing can be purchased for the ApHC.) The stallion owner and the mare owner must file the necessary paperwork within 30 days of insemination.

Other registries may be different, so it is important to look into the requirements in advance.

Keys To Success
The keys to successful AI include having skilled veterinarians or technicians, good communication, and healthy animals. One must have confidence in one's veterinarian. If both veterinarians involved have experience with AI, and a good working knowledge of equine reproduction, then the collection procedure, testing and preparing the mare, and the insemination should go smoothly.

All parties must communicate clearly and promptly for success. All arrangements, such as the approximate date of semen shipment, method of shipment, and the breeding contract, must be made in advance. All parties must be aware of the breed registry requirements. The mare owner should discuss the whole procedure ahead of time with the veterinarian. The exact date of ovulation must be determined, and clearly communicated, to everybody involved.

For AI, as in any breeding, both mare and stallion should be healthy. Prior to breeding, both animals should be examined by the veterinarian for reproductive health, as well as general health. All vaccinations must be up-to-date (especially the mare's rabies vaccination, which can not be given during pregnancy). It is a good idea to have the mare's teeth floated in advance, in order to avoid sedation during pregnancy.

Once the mare is pregnant, there is no difference from any other pregnancy. The mare can be tested by ultrasound or palpation, and cared for according to the usual schedule for a pregnant mare. Pregnancy and delivery will follow its normal course, the same as with natural breeding.

Stallion owner Cheri Moats of Kismet Farm (home of Wap Spotted) is extremely helpful in explaining all aspects of the procedure. She is happy to help stallion owners and mare owners alike to understand the shipped-semen process in layman's terms.

Kismet Farm also has a very informative web site where one can find answers to most of the questions commonly asked by mare owners. You can visit it at: http://www.wapspotted.com.

When asked if she would recommend that other stallion owners provide transported semen, Cheri replied, "Oh yes! Transported semen makes management in general easier and less risky for stallion, mare, handlers, and newborn foals."

Candace Brown of Sapelo Appaloosas, breeder and owner of Sapelo's FB Tucker, also recommends the procedure. She says that, assuming all goes will and the mare settles, "it's almost like spring vaccinations result in a foal!" She recommends that the mare be well-fed (5% over fit weight), vet-checked, and up-to-date on vaccinations and floating before breeding. When asked if she would breed again, using transported semen, she responded, "Of course! A thousand times over!"

Artificial insemination is becoming more and more technologically advanced. With the new millennium, we should think about using the technology to our advantage. Like the "Information Super-Highway," AI is making the world a smaller place, and this can only help us with our goal to save the Appaloosa. We don't have to be limited by geography any more. We can look farther afield for breeding options, using artificial insemination as a tool to widen the horizons of our breeding programs and to improve the fate of the breed we all love.